Медицинская иммунология (Aug 2016)

STUDIES OF INNATE IMMUNITY PARAMETERS (TLR2, TLR4 AND HBD1) OF URETHERAL MUCOUS EPITHELIUM IN CHRONIC BACTERIAL CYSTITIS

  • O. A. Svitich,
  • L. V. Gankovskaya,
  • A. V. Berenshtein,
  • T. S. Perepanova,
  • E. M. Volkova

DOI
https://doi.org/10.15789/1563-0625-2016-5-469-474
Journal volume & issue
Vol. 18, no. 5
pp. 469 – 474

Abstract

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The study for quantitation of TLR2, TLR4 and HBD-1 gene expression in urethral mucosal epithelium from women with chronic bacterial cystitis and healthy females. Materials and methods: RNA was extracted from the samples of urethral mucosa followed by RT-PCR. Results: increased expression of TLR2, TLR4 (respectively, 6.9-, 2.6-fold), along with 13.6-fold decrease in HBD-1 was revealed in women with chronic bacterial cystitis, as compared with appropriate parameters in normal women. Six months later, after combined therapy with autologous immunopeptide complex, TLR2, TLR4 и HBD1 expression levels proved to be near-normal in the patients, being similar to those in healthy females. Conclusion: Imbalanced expression of genes controlling innate immunity was revealed in urethral mucosa of the patients with chronic cystitis, thus increasing risk for urinary tract infections.

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