MONOCYTES AND GRANULOCYTES LIPOPOLYSACCHARIDE-CONNECTING POTENTIAL AT GRAVE'S DISEASE PATIENTS IN INTERRELATION WITH C(-159)T POLYMORPHISM OF CD14 MONOCYTES RECEPTORS

Abstract

The aim of this work was studying of monocytes and granulocytes lipopolysaccharide (LPS)-connecting potential at Grave’s disease patients with various response type on endotoxin in interrelation with C(-159)T polymorphism of CD14 monocytes receptors. The antiendotoxin Ig G (anti-ET-IgG) blood concentrations were studied by ELISA for subdivided group patients according antibodies response to ET. The monocytes and granulocytes LPS-connecting potential were assessed by laser double staining flow cytometry. Genetic polymorphism defined by PCR. All patients with GD were divided into three groups. 1st group included 30 patients ET antibodies hyper- and normoresponders with CC allele combination; the 2nd group was made by 36 patients ET antibodies hyper- and normoresponders with pathological T allele; the 3d group included all ET antibodies hyporesponders (21 patients) who had pathological T allele. 33 healthy donors were investigated too. It was revealed that the monocytes and granulocytes LPS-connecting potential in the group normo-, hyper-and hyporesponders with a genotype, which contains T alel were higher than in group of patients without a mutation and in group of control. Thus patients with hyporesponder response, being combined with a genotype containing T alel in 159 positions of a gene of CD14 receptor, are more predisposed to endotoxin's pathological action.

Keywords

• диффузный токсический зоб
• генетический полиморфизм
• антитела
• рецепторы
• grave's disease
• genetic polymorphism
• antibodies
• receptors