Study of Chronic Toxicity of Strawberry Garden (<i>Fragaria ananassa</i>) Leaves of Dry Extract

Abstract

Introduction. Strawberry garden leaf extract dry (strawberry extract) is one of the potential substances for obtaining safe medicines for the treatment of diseases of the urinary tract, as it has diuretic and anti–inflammatory activity. The study of chronic toxicity is a mandatory step in the preclinical assessment of the safety of a new pharmacological substance.Aim. Preclinical studies of the chronic toxicity of the substance – strawberry extract.Materials and methods. The object of the study was strawberry extract obtained at JSC "Biohimmash". The study of the chronic toxicity of strawberry extract with prolonged administration to rats was carried out in the Laboratory of Medicinal Toxicology of the All-Russian Research Institute of Medicinal and Aromatic Plants in accordance with the "Guidelines for Experimental (Preclinical) Study of new pharmacological Substances" [1]. Laboratory animals were divided into three groups of 15 rats each: group I – control (water); group II – strawberry extract at a dose of 72 mg/kg; group III – strawberry extract – 720 mg/kg.Results and discussion. As a result of a 3-month experiment, it was found that strawberry extract with intragastric administration does not cause the death of animals and does not have a general toxic effect. Also, strawberry extract with intragastric application shows a stable diuretic effect and its preservation during a three-month experiment.Conclusion. Thus, the chronic toxicity of strawberry extract was studied. It was found that strawberry extract does not have a general toxic effect. The conducted research shows the prospects for further research on the development and production of medicines from strawberry leaves with dry extract, which have diuretic and anti-inflammatory activity.

Keywords

• strawberry garden leaf extract dry
• chronic toxicity
• male rats