Техника и технология пищевых производств (Jun 2016)
CLUSTER SILVER INFLUENCE ON PATHOGENIC MICROFLORA OF AGRO-INDUSTRIAL ORGANIC WASTE
Abstract
It is known that the use of silver-containing preparations as anti-pathogenic agents is widely applied in many industries. The paper deals with domestic and foreign experience of cluster silvers application against pathogenic and conventionally pathogenic microflora inhabiting the organic agricultural waste. The main mechanisms of stable cluster silver effect on bacterial cells and changes occurring in them are presented. The present study aims to evaluate the biocidal properties of cluster silver against pathogenic and conventionally pathogenic test cultures: Salmonella typhimurium ATCC 1353, Salmonella pullorum ATCC 19945, Staphylococcus aureus ATCC 25923, Escherichia coli B-5, Proteus vulgaris ATCC 13315, Pseudomonas aeruginosa ATCC 27853 , representing the natural microflora of organic agricultural waste. The results of comparing the effectiveness of cluster silver application and that of antibiotics (amoxicillin, tetracycline, streptomycin, chloramphenicol) to inhibit the growth of Salmonella typhimurium ATCC 1353, Salmonella pullorum ATCC 19945, Staphylococcus aureus ATCC 25923, Escherichia coli B-5, Proteus vulgaris ATCC 13315, Pseudomonas aeruginosa ATCC 27853 are presented. It has been found that cluster silver with 50 ug / ml concentration and above is capable of inhibiting the growth of test cultures at 1.5-2.0 times stronger than antibiotics are. The ability of various concentrations of cluster silver to inhibit the growth and development of bacteria in artificially contaminated substrates by the example of chicken manure has been determined. The bacteriostatic effect of cluster silver against gram-negative Escherichia coli B-5, Proteus vulgaris ATCC 13315 and Pseudomonas aeruginosa ATCC 27853 at a concentration of 80 ug/ml and higher has been established.