STAR Protocols (Sep 2021)

Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions

  • Christoph J. Burckhardt,
  • John D. Minna,
  • Gaudenz Danuser

Journal volume & issue
Vol. 2, no. 3
p. 100644

Abstract

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Summary: Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol presents an optimized co-IP workflow with an analysis based on semi-quantitative immunoblot densitometry to increase reliability and reproducibility.For complete details on the use and execution of this protocol, please refer to Burckhardt et al. (2021).

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