Inhibition of tyrosinase activity and melanine pigmentation by 2-hydroxytyrosol

Abstract

2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L. Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

Keywords

• 2-Hydroxytyrosol
• Metarhizium sp.
• Tyrosinase inhibitor
• Melanine formation
• B16 melanoma cells