A New Meroterpene, A New Benzofuran Derivative and Other Constituents from Cultures of the Marine Sponge-Associated Fungus <i>Acremonium persicinum</i> KUFA 1007 and Their Anticholinesterase Activities
Ana J. S. Alves,
José A. Pereira,
Tida Dethoup,
Sara Cravo,
Sharad Mistry,
Artur M. S. Silva,
Madalena M. M. Pinto,
Anake Kijjoa
Affiliations
Ana J. S. Alves
Laboratório de Química Orgânica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
José A. Pereira
<i>ICBAS</i>-Instituto de Ciências Biomédicas Abel Salazar, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Tida Dethoup
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10240, Thailand
Sara Cravo
Laboratório de Química Orgânica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Sharad Mistry
Department of Chemistry, University of Leicester, University Road, Leicester LE 7 RH, UK
Artur M. S. Silva
Departamento de Química & QOPNA, Universidade de Aveiro, 3810-193 Aveiro, Portugal
Madalena M. M. Pinto
Laboratório de Química Orgânica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Anake Kijjoa
<i>ICBAS</i>-Instituto de Ciências Biomédicas Abel Salazar, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Previously unreported meroterpene, acremine S (1), and benzopyran derivative, acremine T (2), were isolated, together with lumichrome (3), ergosterol (4) and ergosterol 5,8-endoperoxide, from cultures of the marine sponge-associated fungus Acremonium persicinum KUF1007. The structure of the previously unreported compounds was established based on an extensive analysis of 1D and 2D NMR spectra as well as HRMS data. The absolute configurations of the stereogenic centers of 1 were established, unambiguously, based on NOESY correlations and comparison of calculated and experimental electronic circular dichroism (ECD) spectra. Compounds 1−3 were tested for their in vitro acetylcholinesterase and butyrylcholinesterase inhibitory activities.
