KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export

Yan-ru Cui; Shao-jie Wang; Tiancheng Ma; Peihong Yu; Jun Chen; Taijie Guo; Genyi Meng; Biao Jiang; Jiajia Dong; Jia Liu

doi:10.1038/s42003-022-03188-0

Communications Biology (Mar 2022)

KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export

Yan-ru Cui,
Shao-jie Wang,
Tiancheng Ma,
Peihong Yu,
Jun Chen,
Taijie Guo,
Genyi Meng,
Biao Jiang,
Jiajia Dong,
Jia Liu

Affiliations

Yan-ru Cui: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Shao-jie Wang: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Tiancheng Ma: Key Laboratory of Organofluorine Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Peihong Yu: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Jun Chen: College of Life Sciences, Zhejiang University
Taijie Guo: Key Laboratory of Organofluorine Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Genyi Meng: Key Laboratory of Organofluorine Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Biao Jiang: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Jiajia Dong: Key Laboratory of Organofluorine Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Jia Liu: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University

DOI: https://doi.org/10.1038/s42003-022-03188-0
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

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The FDA-approved anti-cancer drug, KPT330, can indirectly inhibit Cas9 by interfering with Cas9 mRNA nuclear export and help reduce off-target editing in cells.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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