Gut microbiota-derived metabolites associate with circulating immune cell subsets in unexplained recurrent spontaneous abortion
Zhi Li,
Yongquan Zheng,
Meng Zhang,
Kaiqi Wu,
Long Zhang,
Yao Yao,
Caihong Zheng
Affiliations
Zhi Li
Department of Gynaecology and Obstetrics, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China
Yongquan Zheng
Department of Pharmacy, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China
Meng Zhang
Department of Pharmacy, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China
Kaiqi Wu
Department of Clinical Laboratory, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China
Long Zhang
Department of Clinical Laboratory, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China
Yao Yao
Department of Pharmacy, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China; Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, China; Corresponding author. No. 1 Xueshi Road, Shangcheng District, Hangzhou, 310006, China.
Caihong Zheng
Department of Pharmacy, Women's Hospital School of Medicine Zhejiang University, Hangzhou, 310006, China; Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, China; Corresponding author. No. 1 Xueshi Road, Shangcheng District, Hangzhou, 310006, China.
Currently, the precise causes of over 40 % of recurrent spontaneous abortion (RSA) cases cannot be identified, leading to the term “unexplained RSA” (URSA). Through an exploration of the gut microbiota, metabolites, and immune cell subsets in URSA, this study establishes a link between gut microbiota-derived metabolites and immune cells. The results indicate reduced diversity in the gut microbiota of URSA. Targeted metabolomic analyses reveal decreased levels of gut microbiota-derived deoxycholic acid (DCA), glycolithocholic acid (GLCA), acetate, propionate, and butyrate in URSA. Furthermore, elevated frequencies of Th1, Th17, and plasma B cells, along with decreased frequencies of Tregs and Bregs, are observed in the peripheral blood of URSA. The results demonstrate correlations between the levels of gut microbiota-derived bile acids and short-chain fatty acids and the frequencies of various immune cell subsets in circulation. Collectively, this study uncovers an association between gut microbiota-derived metabolites and circulating immune cell subsets in URSA.
