Rapid extraction and detection of ellagic acid in plant samples using a selective magnetic molecularly imprinted polymer coupled to a fluorescence method

Ouarda El Hani; Abdelhafid Karrat; Juan José García-Guzmán; José María Palacios-Santander; Khalid Digua; Aziz Amine; Laura Cubillana-Aguilera

Green Analytical Chemistry (Jun 2023)

Rapid extraction and detection of ellagic acid in plant samples using a selective magnetic molecularly imprinted polymer coupled to a fluorescence method

Ouarda El Hani,
Abdelhafid Karrat,
Juan José García-Guzmán,
José María Palacios-Santander,
Khalid Digua,
Aziz Amine,
Laura Cubillana-Aguilera

Affiliations

Ouarda El Hani: Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, P.A. 149., Mohammedia, Morocco; Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain
Abdelhafid Karrat: Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, P.A. 149., Mohammedia, Morocco; Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain
Juan José García-Guzmán: Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain
José María Palacios-Santander: Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain; Corresponding author.
Khalid Digua: Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, P.A. 149., Mohammedia, Morocco
Aziz Amine: Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, P.A. 149., Mohammedia, Morocco
Laura Cubillana-Aguilera: Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain

Journal volume & issue: Vol. 5
p. 100058

Abstract

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A remarkable growth was noticed in the development of molecularly imprinted polymers (MIPs), which have used as efficient synthetic antibodies that contain selective cavities to the target molecule. Hereunder, a novel strategy using MIP as a sorbent in solid-phase extraction was coupled to a fluorescence method for ellagic acid (EA) purification and immediate detection. The synthesis of magnetic-MIP (MMIP) using a rapid and green ultrasound technology was assessed by central composite design to determine the optimal polymerization conditions for a high-imprinting polymer. The MMIP was characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and scanning/transmission electron microscopy, which accurately confirmed the functional, magnetic, and morphological features of MMIP. The prepared MMIP was demonstrated to be selective for EA compared to many similar phenols. The spectrofluorometric method showed a linear range from 0.05 to 2 μg·mL−1 of EA, and the limits of detection (LOD) and quantification (LOQ) were 0.005 and 0.02 μg·mL−1, respectively. Besides, the novel proposed smartphone method using the ultraviolet lamp as the excitation source presented a linear range from 0.2 to 4 μg·mL−1, a LOQ of 0.2 μg·mL−1, and a LOD of 0.07 μg.mL−1. The proposed strategy revealed high efficiency in the extraction and detection of EA in grape, redberry, and green tea. Effectively, the calculated recoveries were ranging from 80 to 102% with low values of relative standard deviation (<3%). The proposed strategy could be used in many analytical fields for selective, rapid, user-friendly, visual, and cost-effective detection.

Published in Green Analytical Chemistry

ISSN: 2772-5774 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry: Analytical chemistry
Website: https://www.sciencedirect.com/journal/green-analytical-chemistry

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