Polyoxypregnane Ester Derivatives and Lignans from <i>Euphorbia gossypina</i> var. <i>coccinea</i> Pax.
Reham Hammadi,
Norbert Kúsz,
Csilla Zsuzsanna Dávid,
Peter Waweru Mwangi,
Róbert Berkecz,
Nikoletta Szemerédi,
Gabriella Spengler,
Judit Hohmann,
Andrea Vasas
Affiliations
Reham Hammadi
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary
Norbert Kúsz
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary
Csilla Zsuzsanna Dávid
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary
Peter Waweru Mwangi
School of Biological Sciences, University of Nairobi, P.O. Box 30197, Nairobi 00100, Kenya
Róbert Berkecz
Institute of Pharmaceutical Analysis, University of Szeged, 6720 Szeged, Hungary
Nikoletta Szemerédi
Department of Medical Microbiology, Albert Szent-Györgyi Health Center, Albert Szent-Györgyi Medical School, University of Szeged, 6725 Szeged, Hungary
Gabriella Spengler
Department of Medical Microbiology, Albert Szent-Györgyi Health Center, Albert Szent-Györgyi Medical School, University of Szeged, 6725 Szeged, Hungary
Judit Hohmann
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary
Andrea Vasas
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary
From the aerial parts of Euphorbiagossypina var. coccinea Pax., eight new pregnane glycosides (euphogossypins A–H, 1–8) of the cynanforidine and deacetylmetaplexigenin aglycons, two new lignans (gossypilignans A and B, 9 and 10), and four known compounds, namely, the pregnane 12-O-benzoyldeaxcylmetaplexigenin (11), the lignan 9α-hydroxypinoresinol (12), and the flavonoids naringenin (13) and quercitrin (14) were isolated. The structure elucidation of the new compounds was carried out by a spectroscopic analysis, including HRMS, 1D (1H, 13C JMOD), and 2D NMR (HSQC, 1H–1H COSY, HMBC, and NOESY) experiments. The obtained pregnane glycosides were substituted with acetyl and benzoyl ester moieties, and sugar chains containing thevetose, cymarose, digitoxose, and glucose monosaccharides. All of the compounds are described for the first time from E. gossypina var. coccinea. The isolated pregnanes and lignans were tested for their antiproliferative activity on HeLa cells using the MTT assay; the compounds exerted no significant effect against the tumor cells.