Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice
Leonardo Mendes de Souza Mesquita,
Cíntia Rabelo e Paiva Caria,
Paola Souza Santos,
Caio Cesar Ruy,
Natalia da Silva Lima,
Débora Kono Taketa Moreira,
Claudia Quintino da Rocha,
Daniella Carisa Murador,
Veridiana Vera de Rosso,
Alessandra Gambero,
Wagner Vilegas
Affiliations
Leonardo Mendes de Souza Mesquita
UNESP—São Paulo State University/Coastal Campus of São Vicente, Laboratory of Bioprospection of Natural Products (LBPN) Pça Infante Dom Henrique S/N, 11330-900 São Vicente, SP, Brazil
Cíntia Rabelo e Paiva Caria
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Paola Souza Santos
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Caio Cesar Ruy
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Natalia da Silva Lima
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Débora Kono Taketa Moreira
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Claudia Quintino da Rocha
UNESP—São Paulo State University/Coastal Campus of São Vicente, Laboratory of Bioprospection of Natural Products (LBPN) Pça Infante Dom Henrique S/N, 11330-900 São Vicente, SP, Brazil
Daniella Carisa Murador
Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim Street, 136, Vila Mathias, 11015-020 Santos City, SP, Brazil
Veridiana Vera de Rosso
Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim Street, 136, Vila Mathias, 11015-020 Santos City, SP, Brazil
Alessandra Gambero
Clinical Pharmacology and Gastroenterology Unit, USF—São Francisco University, Av. São Francisco de Assis, 218, 12916-900 Bragança Paulista, SP, Brazil
Wagner Vilegas
UNESP—São Paulo State University/Coastal Campus of São Vicente, Laboratory of Bioprospection of Natural Products (LBPN) Pça Infante Dom Henrique S/N, 11330-900 São Vicente, SP, Brazil
No scientific report proves the action of the phytochemicals from the mangrove tree Rhizophora mangle in the treatment of diabetes. The aim of this work is to evaluate the effects of the acetonic extract of R. mangle barks (AERM) on type 2 diabetes. The main chemical constituents of the extract were analyzed by high-performance liquid chromatography (HPLC) and flow injection analysis electrospray-iontrap mass spectrometry (FIA-ESI-IT-MS/MS). High-fat diet (HFD)-fed mice were used as model of type 2 diabetes associated with obesity. After 4 weeks of AERM 5 or 50 mg/kg/day orally, glucose homeostasis was evaluated by insulin tolerance test (kiTT). Hepatic steatosis, triglycerides and gene expression were also evaluated. AERM consists of catechin, quercetin and chlorogenic acids derivatives. These metabolites have nutritional importance, obese mice treated with AERM (50 mg/kg) presented improvements in insulin resistance resulting in hepatic steatosis reductions associated with a strong inhibition of hepatic mRNA levels of CD36. The beneficial effects of AERM in an obesity model could be associated with its inhibitory α-amylase activity detected in vitro. Rhizophora mangle partially reverses insulin resistance and hepatic steatosis associated with obesity, supporting previous claims in traditional knowledge.
