Sensors (Jul 2014)

Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents

  • Daniel Matatagui,
  • José Luis Fontecha,
  • María Jesús Fernández,
  • Isabel Gràcia,
  • Carles Cané,
  • José Pedro Santos,
  • María Carmen Horrillo

DOI
https://doi.org/10.3390/s140712658
Journal volume & issue
Vol. 14, no. 7
pp. 12658 – 12669

Abstract

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The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.

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