Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Mangga Arumanis (Mangifera indica L.) dan Identifikasi Flavonoid dengan KLT

Abstract

Arumanis mango leaves (Mangifera indica L.) has antibacterial activity and antifungal, evidently. Escherichia coli and staphylococcus aureus are opprtunistic pathogen bacteria that can cause gastrointestinal and skin infection. Purification of extract was done to eliminate the presence of ballast substances that cannot produce therapeutic effects. This study aims to acknowledge the antibacterial activity of purified mango arumanis leaves extract (PMALE) against E. coli and S. aureus, flavonoid compound content by TLC. Arumanis Mango leaves powder was extracted with 96% ethanol solvent by maceration method, followed purification the extract was done with hot water-ethylacetate solvent by liquid-liquid extraction method. Antibacterial activity test was done by agar diffusion method against E. coli and S. aureus bacteria with the same serial concentration of 6.25; 12.5; 25; 50; 75 and 100% and Thin Layer Chromatography (TLC) tests for detection flavonoid compounds, the stationary phase used silica gel 60F 254 and the mobile phase used n-hexane-ethylacetate (1:3). The results of PMALE had antibacterial activity at lower concentrations 6,25% against both of E. coli and S. aureus. PMALE had flavonoid compounds with Rf values of 0.81.

Keywords

• arumanis mango leaves
• mangifera indica l.
• purified extract
• antibacterial
• flavonoid
• thin layer chromatography