Applied Sciences (Aug 2020)

Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of <i>Vibrio parahaemolyticus</i>

  • Miki Maetani-Yasui,
  • Kazuaki Mawatari,
  • Airi Honjo,
  • Thi Kim Ngan Bui,
  • Takaaki Shimohata,
  • Takashi Uebanso,
  • Mutsumi Aihara,
  • Takahiro Emoto,
  • Masatake Akutagawa,
  • Yohsuke Kinouchi,
  • Akira Takahashi

DOI
https://doi.org/10.3390/app10165549
Journal volume & issue
Vol. 10, no. 16
p. 5549

Abstract

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Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.

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