Comparative study of polyphenolic compound extraction from empty palm fruit bunches and sugarcane pulp

Abstract

Polyphenolic compounds have many benefits, one of which being their efficacy as antioxidants. They can be extracted from various parts of plants and from agricultural waste. In this research, sugarcane pulp, and empty palm fruit bunches from the palm oil production were investigated as potential raw materials. This study aims to determine solvents and easy-to-perform extraction methods that show the highest effectivity in regards to total phenolic and flavonoid yield and the correlated antioxidant activity. Extraction methods comprised maceration, Soxhlet extraction, and ultrasound assisted extraction (UAE); solvents that were investigated included water, 70% methanol and 70% ethanol. The antioxidant activity was measured by the DPPH (diphenyl-2-picrylhydrazyl) method and FRAP (Ferric Reduction Ability of Plasma) method. Based on the amount of polyphenol compounds as well as the antioxidant activity, the experiments showed that Soxhlet extraction with 70% methanol as solvent worked best for palm bunch waste and sugar cane pulp, resulted in about two times higher values for total phenolic content, flavonoid content and FRAP antioxidant activity as well as extract mass (yield) compared to the results from other extraction methods or solvents used in this experiment. The antioxidant activity of the extracts as measured by DPPH method seemed also to be promising, although the trend among solvent and extraction method was rather inconclusive.

Keywords

• Antioxidants
• Empty palm fruit bunches
• Extraction
• Sugarcane pulp