Nature Communications (Sep 2021)

In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli

  • Philipp S. Erdmann,
  • Zhen Hou,
  • Sven Klumpe,
  • Sagar Khavnekar,
  • Florian Beck,
  • Florian Wilfling,
  • Jürgen M. Plitzko,
  • Wolfgang Baumeister

DOI
https://doi.org/10.1038/s41467-021-25413-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 9

Abstract

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The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome maturation and reveal the native organization of the nucleolus.