Nature Communications (Sep 2021)
In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli
Abstract
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome maturation and reveal the native organization of the nucleolus.