Trichoderma harzianum Root Colonization in Arabidopsis
Ana Alonso-Ramírez,
Jorge Poveda,
Ignacio Martín,
Rosa Hermosa,
Enrique Monte,
Carlos Nicolás
Affiliations
Ana Alonso-Ramírez
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca, Salamanca, Spain
Jorge Poveda
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca, Salamanca, Spain
Ignacio Martín
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca, Salamanca, Spain
Rosa Hermosa
Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain
Enrique Monte
Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain
Carlos Nicolás
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca, Salamanca, Spain
Trichoderma is a soil-borne fungal genus that includes species with a significant impact on agriculture and industrial processes. In this article we show a detailed protocol of Trichoderma harzianum (T. harzianum) root invasion procedure described by Alonso-Ramírez et al. (2014). Some Trichoderma strains exert beneficial effects in plants through root colonization. They promote growth and development, modify root architecture, facilitate efficient nutrient use, or stimulate defenses against pathogens, although little is known about how this interaction takes place. For this purpose, Trichoderma-Arabidopsis hydroponic cultures were grown inside Phytatray II boxes, using mycelia obtained from spores of T. harzianum and Arabidopsis thaliana (A. thaliana) seedlings. In this way changes in root architecture, such as callose deposition, promoted by the fungus can be analyzed.
