The Potential Action of Thomsen–Friedenreich Monoclonal Antibody (A78-G/A7) in Thyroid Cancer

Abstract

Ying Peng, 1 Xiang-Xiang Zhan, 2 Yi Cao, 3 Han-Wen Zhang, 1 Wei-Han Cao, 1, 2 Yan-Jun Su, 1, 2 Chang Diao, 2 Qiang-Ming Sun, 1, 2, 4, 5 Ruo-Chuan Cheng 1, 2, 4, 5 1Kunming Medical University of Yunnan Province, Kunming, Yunnan 650500, People’s Republic of China; 2Thyroid Disease Diagnosis and Treatment Center, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, People’s Republic of China; 3Longyan Jianhai Medical and Pharmaceutical Technology Co., Ltd., Longyan, Fujian 364000, People’s Republic of China; 4Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, People’s Republic of China; 5Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, People’s Republic of ChinaCorrespondence: Ruo-Chuan Cheng; Qiang-Ming Sun Email [email protected]; [email protected]: Thomsen–Friedenreich antibody (TF-Ab) is a specific antibody against the Thomsen–Friedenreich antigen (TF-Ag). At present, studies on a number of other tumors have shown that TF-Ab can effectively inhibit metastasis and induce apoptosis in tumor cells. However, the role of TF-Ab in thyroid cancer (TC) remains unclear.Materials and Methods: Normal subjects and patients with primary papillary TC with or without lymph node metastasis were tested for TF-Ab expression by enzyme-linked immunosorbent assays (ELISAs). Immunofluorescence was used to assess the expression of TF-Ag in thyroid papillary carcinoma with or without lymph node metastasis and undifferentiated cancer tissues. To evaluate the role of TF-Ab in TC, the effects of TF monoclonal antibody (mAb A78-G/A7) on cell biological function were investigated by MTT assays, flow cytometry, adhesion assays and transwell experiments.Results: Compared with normal individuals, TF-Ab levels in patients with TC were decreased, but no changes were observed with respect to lymph node metastasis. The expression of TF-Ag in TC tissues was relatively higher than that detected in adjacent tissues, but it was not affected by the presence or absence of lymph node metastasis. Upon treatment mAb A78-G/A7 treating, TC cell cycles were affected, meanwhile the abilities to adhere, invade and migrate were also significantly reduced.Conclusion: The results of the present study showed that mAb A78-G/A7 could affect the invasion and migration of all assayed TC cell lines. The effects of mAb A78-G/A7 on the cell cycle, adhesion, invasion and migration of TC cells were more significant than those observed for proliferation and apoptosis.Keywords: Thomsen–Friedenreich antibody, TF-Ab, Thomsen–Friedenreich antigen, TF-Ag, mAb A78-G/A7, thyroid cancer, TC

Keywords

• thomsen-friedenreich antibody(tf-ab)
• thomsen-friedenreich antigen(tf-ag)
• mab a78-g/a7
• thyroid cancer (tc)