Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)

Dangfeng Wang; Dangfeng Wang; Dangfeng Wang; Fangchao Cui; Fangchao Cui; Likun Ren; Xiqian Tan; Xiqian Tan; Xinran Lv; Xinran Lv; Qiuying Li; Qiuying Li; Jianrong Li; Jianrong Li; Jianrong Li; Tingting Li

doi:10.3389/fmicb.2022.856802

Frontiers in Microbiology (Apr 2022)

Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)

Dangfeng Wang,
Dangfeng Wang,
Dangfeng Wang,
Fangchao Cui,
Fangchao Cui,
Likun Ren,
Xiqian Tan,
Xiqian Tan,
Xinran Lv,
Xinran Lv,
Qiuying Li,
Qiuying Li,
Jianrong Li,
Jianrong Li,
Jianrong Li,
Tingting Li

Affiliations

Dangfeng Wang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Dangfeng Wang: College of Food Science and Technology, Bohai University, Jinzhou, China
Dangfeng Wang: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Fangchao Cui: College of Food Science and Technology, Bohai University, Jinzhou, China
Fangchao Cui: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Likun Ren: Key Laboratory of Food Science and Engineering of Heilongjiang Province, College of Food Engineering, Harbin University of Commerce, Harbin, China
Xiqian Tan: College of Food Science and Technology, Bohai University, Jinzhou, China
Xiqian Tan: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Xinran Lv: College of Food Science and Technology, Bohai University, Jinzhou, China
Xinran Lv: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Qiuying Li: College of Food Science and Technology, Bohai University, Jinzhou, China
Qiuying Li: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Jianrong Li: School of Food Science and Technology, Jiangnan University, Wuxi, China
Jianrong Li: College of Food Science and Technology, Bohai University, Jinzhou, China
Jianrong Li: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, China
Tingting Li: Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian, China

DOI: https://doi.org/10.3389/fmicb.2022.856802
Journal volume & issue: Vol. 13

Abstract

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Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of P. fluorescens PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of P. fluorescens PF08. Results of quantitative real-time PCR revealed the key role of the P. fluorescens PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of P. fluorescens PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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