A pH-sensitive liposomal co-delivery of fingolimod and ammonia borane for treatment of intracerebral hemorrhage

Abstract

Intracerebral hemorrhage (ICH) is one of the most devastating types of stroke. This study aims to develop a new drug carrier with hematoma-specific response and high property. pH-sensitive liposomes (PSL) were developed. Fingolimod with ammonia borane were encapsulated in the phospholipid vesicles to integrate two drugs for treating ICH more effectively. pH sensitive PSL-FTY720/AB was characterized for various physicochemical parameters such as shape, surface morphology, vesicle size, zeta-potential, in-vitro drug release in different pH environment, cellular toxicity, in-vivo and ex-vivo tissue accumulation. In vivo results further confirmed that drug-loading nanoparticles effectively protected against ICH-induced brain injury through synergistic effect of anti-inflammation and anti-oxidation. Collectively, the present study confirmed that PSL-FTY720/AB can be an effective, safe, and a novel alternative treatment approach in ICH.

Keywords

• ammonia borane
• fingolimod
• intracerebral hemorrhage
• liposome