Dental Research Journal (Jan 2012)

Microflora and periodontal disease

  • Luca Scapoli,
  • Ambra Girardi,
  • Annalisa Palmieri,
  • Tiziano Testori,
  • Francesco Zuffetti,
  • Riccardo Monguzzi,
  • Dorina Lauritano,
  • Francesco Carinci

DOI
https://doi.org/10.4103/1735-3327.109755
Journal volume & issue
Vol. 9, no. 8
pp. 202 – 206

Abstract

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Background: Periodontitis is a disease that affects and destroys the tissues that support teeth. Tissue damage results from a prolonged inflammatory response to an ecological shift in the composition of subgingival biofilms. Three bacterial species that constitute the red complex group, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, are considered the main pathogens involved in periodontitis. Materials and Methods: In the present study, a real-time polymerase chain reaction bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results: Significant higher prevalence of red complex species and increased amount of P. gingivalis and T. denticola were detected in periodontal pocket of periodontitis patients. Conclusions: Results demonstrated that the test is a valuable tool to improve diagnosis of periodontal disease.

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