OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition
Ruina Zhang,
Mengtian Pei,
Shiyi Lin,
Jing Chen,
Jules Biregeya,
Linlin Song,
Changlin Peng,
Pengcheng Jiang,
Guo-dong Lu
Affiliations
Ruina Zhang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Mengtian Pei
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Shiyi Lin
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Jing Chen
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Jules Biregeya
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Linlin Song
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Changlin Peng
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Pengcheng Jiang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Guo-dong Lu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University
Abstract Phosphatidylinositol signaling system plays a crucial role in plant physiology and development, phosphatidylinositol phosphate kinases (PIPKs) are one of the essential enzymes responsible for catalyzing the synthesis of phosphatidylinositol bisphosphate (PIP2) within this signaling pathway. However, its mechanism of signal transduction remains poorly exploited in plants. OsMBL1, a jacalin-related mannose-binding lectin in rice, plays a crucial role in plant defense mechanisms, acting as a key component of the pattern-triggered immunity (PTI) pathway. Here, a rice phosphatidylinositol-phosphate kinase FAB (OsPIPK-FAB), a member of the rice PIPKs family, as an interacting protein of OsMBL1 through yeast-two-hybrid (Y2H) screening assay. And this interaction was confirmed by using co-immunoprecipitation (Co-IP) and pull-down assay techniques. Furthermore, we demonstrated that the deletion of OsPIPK-FAB gene in plant enhanced resistance against rice blast while overexpression of OsPIPK-FAB increases sensitivity to the fungal infection. Additionally, through determination and measurement of the plant inositol 1,4,5-trisphosphate (IP3) contents and the plant phosphatidylinositol 4-phosphate 5-kinase (PIP5K) activity, we revealed that OsMBL1 inhibits the PIP5K kinase activity of OsPIPK-FAB as well as the plant IP3 contents in rice. Conclusively, these findings indicated that OsPIPK-FAB serves as a novel and critical component that is negatively involved in PTI activation and was inhibited by OsMBL1.
