Entomopathogenicity of Simplicillium lanosoniveum CG888 on Mortality of Callosobruchus maculatus F. in vitro

Abstract

Research regarding Simplicillium lanosoniveum as an entomopathogen is still limited. The entomopathogenicity of Simplicillium lanosoniveum CG888 with three different conidia density treatments, namely 1x106/ml, 1x107/ml, and 1x108/ml, was tested on Callosobruchus maculatus F. imago in the laboratory, incubated at 26-27°C, RH 70% and in the dark conditions. Based on the effect of conidia density, S. lanosoniveum CG888 at a density of 1x108 conidia/ml caused greater mortality of C. maculatus F, reaching 51.65% compared to other treatments and control. The influence of the length of time after inoculation was that there was 60.83% C. maculatus F. death on the 11th day. It was higher than the result on 3, 5, 7 and 9 days later. There was a positive interaction that the mortality of C. maculatus F was influenced by the density of S. lanosoniveum CG888 conidia and the period of days after inoculation. Among these treatments, S. lanosoniveum CG888 with 1x108/ml caused mortality of 86,7% in C. maculatus on day 11 after inoculation. The lowest mortality of C. maculatus F. was in the density of 1x106 conidia/ml of 70%. These findings indicate that S. lanosoniveum CG888 can be used to control C. maculatus F. safely and reduce the use of chemicals in soybean storage.