Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat
Weiwei Mao,
Xiaobo Wang,
Yongming Chen,
Yongfa Wang,
Liang Ma,
Xiaoming Xie,
Xiaojia Wu,
Jin Xu,
Yuqi Zhang,
Yue Zhao,
Xuejun Tian,
Weilong Guo,
Zhaorong Hu,
Mingming Xin,
Yingyin Yao,
Zhongfu Ni,
Qixin Sun,
Huiru Peng
Affiliations
Weiwei Mao
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Xiaobo Wang
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Yongming Chen
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Yongfa Wang
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Liang Ma
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Xiaoming Xie
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Xiaojia Wu
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Jin Xu
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Yuqi Zhang
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Yue Zhao
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Xuejun Tian
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Weilong Guo
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Zhaorong Hu
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Mingming Xin
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Yingyin Yao
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Zhongfu Ni
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Qixin Sun
Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Huiru Peng
Corresponding author.; Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
Pigment accumulation is an important trait related to wheat domestication, but there remains a limited understanding of its molecular mechanism. The genetic control of the red glume trait by a dominant allele, Rg-B1, on 1BS was reported in the last century, but the underlying gene and its molecular basis remained elusive. Here, we identified TraesTSP1B01G005700 (G57) encoding an R2R3-MYB transcription factor (TF) as the candidate Rg-B1 gene controlling red glume color by a combination of genome-wide association study (GWAS), bulked segregant RNA-sequencing (BSR-Seq), map-based cloning, and RNA-seq. The Rg-B1 locus had zero to five duplicate copies only one of which had high transcriptional activity. Genetic evidence suggested that promoter sequence variation in G57 in the glume leads to high expression of G57, resulting in the red glume phenotype. G57 could bind to the promoters of anthocyanin synthesis genes TaCHS, TaF3ʹH, and TaUFGT, activating their expression and contributing to anthocyanin accumulation in wheat glume. G57 also played a pivotal role in up-regulating expression of genes TaDREB1C and TaFLO2 associated with increased grain weight, thereby causing increased grain weight. Our research offers a better understanding of the molecular basis of red glume in bread wheat.
