Nature Communications (May 2017)

pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4CRBN ubiquitin ligase

  • Jian An,
  • Charles M. Ponthier,
  • Ragna Sack,
  • Jan Seebacher,
  • Michael B. Stadler,
  • Katherine A. Donovan,
  • Eric S. Fischer

DOI
https://doi.org/10.1038/ncomms15398
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

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Targeting therapeutically-relevant proteins for degradation is an emerging paradigm in drug discovery. Here the authors describe a sensitive pulse SILAC mass spectrometry-based proteomics approach that reports global changes in protein stability following drug treatment in a single time point experiment.