A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for <i>Listeria monocytogenes</i> Detection
Liangliang Zhu,
Hongshun Hao,
Chao Ding,
Hanwei Gan,
Shuting Jiang,
Gongliang Zhang,
Jingran Bi,
Shuang Yan,
Hongman Hou
Affiliations
Liangliang Zhu
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Hongshun Hao
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Chao Ding
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Hanwei Gan
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Shuting Jiang
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Gongliang Zhang
Liaoning Key Lab for Aquatic Processing Quality and Safety, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Jingran Bi
Liaoning Key Lab for Aquatic Processing Quality and Safety, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Shuang Yan
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China
Hongman Hou
Liaoning Key Lab for Aquatic Processing Quality and Safety, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 101–1.3 × 107 CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes.
