Oestradiol upregulates the expression of Cyp19a1a and the feminization ratio in Schizothorax prenanti

Abstract

Abstracts: Sex plasticity is easily influenced by external conditions in teleosts. In the present study, the morphogenesis of gonad development was described, and the sex ratio and gonadal development were also analysed after induction by exogenous E2. The primordial gonad was first visible at 9 dph, and PGCs entered mitosis at 90 dph. The time of transition from single oogonia to clusters of oogonia was from 95 dph until 126 dph, and the formation of chromatin nucleolar oocytes appeared at 212 dph. Compared with the ovary, the putative testis had fewer germ cells and a smaller gonad, which started to differentiate at 186 dph and formed spermatocytes at 237 dph. Exogenous E2 treatment promoted the expression of Cyp19a1a in differentiating gonads, and the female rate of all E2-treated groups was significantly higher than control groups (P < 0.05). Taken together, these results will aid in the further analysis of mechanisms underlying sex differentiation in S. prenanti.

Keywords

• Sexual differentiation
• 17β-oestradiol
• Cyp19a1a
• Feminization
• Schizothorax prenanti