Cryopreservation of infectious Cryptosporidium parvum oocysts

Justyna J. Jaskiewicz; Rebecca D. Sandlin; Anisa A. Swei; Giovanni Widmer; Mehmet Toner; Saul Tzipori

doi:10.1038/s41467-018-05240-2

Nature Communications (Jul 2018)

Cryopreservation of infectious Cryptosporidium parvum oocysts

Justyna J. Jaskiewicz,
Rebecca D. Sandlin,
Anisa A. Swei,
Giovanni Widmer,
Mehmet Toner,
Saul Tzipori

Affiliations

Justyna J. Jaskiewicz: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University
Rebecca D. Sandlin: BioMEMS Resource Center, Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospitals for Children
Anisa A. Swei: BioMEMS Resource Center, Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospitals for Children
Giovanni Widmer: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University
Mehmet Toner: BioMEMS Resource Center, Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospitals for Children
Saul Tzipori: Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University

DOI: https://doi.org/10.1038/s41467-018-05240-2
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

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Research on Cryptosporidium parasites, a major cause of acute infant diarrhea, is hampered by the short shelf life of oocysts, thus requiring routine propagation in laboratory animals. Here, the authors report a method to cryopreserve viable and infectious Cryptosporidium oocysts by ultra-fast cooling.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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