CDT1, transcriptionally regulated by E2F2, promotes lung adenocarcinoma progression
Bao-Quan Lin,
Feng Chen,
Lei Gu,
Zai-Xin Wu,
Jia Ye,
Lei Zhang,
Bing-jing Huang,
Zong-yang Yu,
Guo-Xiang Lai,
Xiao-Peng Lan,
Hu Zhao,
Wei Liu
Affiliations
Bao-Quan Lin
Cardio-Thoracic Surgery Department, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Feng Chen
Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China
Lei Gu
Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China
Zai-Xin Wu
Medical Service Management Office, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Jia Ye
Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China; Department of Respiratory and Critical Care Medicine, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Lei Zhang
Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China; Department of Respiratory and Critical Care Medicine, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Bing-jing Huang
Department of Respiratory and Critical Care Medicine, Xiamen Haicang Hospital, No. 89 Haiyu Road, Xiamen, Fujian, 361026, China
Zong-yang Yu
Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China; Department of Respiratory and Critical Care Medicine, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Guo-Xiang Lai
Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China; Department of Respiratory and Critical Care Medicine, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China
Xiao-Peng Lan
Institute for Laboratory Medicine, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fujian Medical University, Fuzhou, Fujian, 350025, China
Hu Zhao
Department of General Surgery, Fuzong Clinical Medical College of Fujian Medical University, 900TH Hospital of Joint Logistics Support Force, Fuzhou, Fujian, 350025, China; Department of General Surgery, Dongfang Hospital of Xiamen University, School of Medicine, Xiamen University, 900TH Hospital of Joint Logistics Support Force, Fuzhou, Fujian, 350025, China; Corresponding author. Department of General Surgery, Fuzong Clinical Medical College of Fujian Medical University, Department of General Surgery, 900TH Hospital of Joint Logistics Support Force, Fuzhou, Fujian, 350025, China.
Wei Liu
Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China; Department of Respiratory and Critical Care Medicine, Fuzong Clinical Medical College of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army, Fuzhou, Fujian, 350025, China; Corresponding author. Department of Respiratory and Critical Care Medicine, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (The 900th Hospital of the Joint Logistic Support Force, People's Liberation Army), Fuzhou, Fujian, 350025, China.
CDT1, a gene that shows excessive expression in various malignancies, functions as a pivotal regulator of replication licensing. In this study, we observed a positive correlation in expression between CDT1 and E2F2 among patients with lung adenocarcinoma (LUAD). Our findings substantiated that E2F2 directly interacted with the promoter region of CDT1, as confirmed by ChIP-qPCR assays, and depletion of E2F2 resulted in a downregulation of CDT1 expression in LUAD cell lines by gene interference technology. Furthermore, we identified an upregulation of CDT1 mRNA level in Chinese LUAD samples. Notably, in the loss-of-function assays, depletion of CDT1 in LUAD cell lines inhibited cell proliferation, migration, and invasion. Concurrently, it promoted cell apoptosis and induced G0/G1 phase arrest using MTT, flow cytometry, and Transwell assays, reinforcing its role as an oncogene.Furthermore, enhanced tumor ablation was determined in a CDT1-downregulated LUAD tumor-bearing nude mouse model. Collectively, our results strongly suggest that E2F2 positively regulates CDT1 expression and actively participates in the progression of lung adenocarcinoma, thereby providing valuable insights into identifying novel therapeutic targets for LUAD treatment.
