Effects of ABT on the morphogenesis and inclusions of Taxus chinensis (Pilger) Rehd f. baokangsis cutting rooting

Abstract

This study aims to explore the cutting propagation method of a novel variant on Taxus chinensis (Pilger) Rehd f. baokangsis (T. chinensis baokangsis). Different types of rooting powder and different concentrations were used to treat the cuttage seedlings of T. chinensis baokangsis, and then the external morphology and anatomical morphology of the roots were observed. The membership function evaluation method was used to evaluate the cutting effect. The physiological characteristics of T. chinensis baokangsis cuttings were studied by the correlation analysis of nutrient components and endogenous hormone content. The results showed that the T. chinensis baokangsis belonged to callus rooting type, and the adventitious roots differentiated at about 150 d. For rooting growth indexes, the optimal treatment was ABT-1+400 mg/L. The rooting rate of T. chinensis baokangsis was positively correlated with the content of soluble sugar, soluble starch, and IAA, while extremely significantly negatively correlated with MDA (P <0.01). Moreover, the rooting rate also was negatively correlated with ABA, ZR, and GA3, and significantly negatively correlated with GA3 (P <0.05). This study will provide some technical support and theoretical basis for the conservation and reproduction of T. chinensis baokangsis.

Keywords

• ABT
• cuttings
• Taxus chinensis (Pilger) Rehd f. baokangsis