Biotechnology Reports (Dec 2019)

Complete genome sequence of Nitratireductor sp. strain OM-1: A lipid-producing bacterium with potential use in wastewater treatment

  • Yoshiko Okamura,
  • Laura Treu,
  • Stefano Campanaro,
  • Sena Yamashita,
  • Shota Nakai,
  • Hirokazu Takahashi,
  • Kenshi Watanabe,
  • Irini Angelidaki,
  • Tsunehiro Aki,
  • Yukihiko Matsumura,
  • Yutaka Nakashimada

Journal volume & issue
Vol. 24

Abstract

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Reducing CO2 emissions is necessary to alleviate rising global temperature. Renewable sources of energy are becoming an increasingly important substitute for fossil fuels. An important step in this direction is the isolation of novel, technologically relevant microorganisms. Nitratireductor sp. strain OM-1 can convert volatile short-chain fatty acids in wastewater into 2-butenoic acid and its ester and can accumulate intracellularly esterified compounds up to 50% of its dried cell weight under nitrogen-depleted conditions. It is believed that a novel fatty acid biosynthesis pathway including an esterifying enzyme is encoded in its genome. In this study, we report the whole-genome sequence (4.8 Mb) of OM-1, which comprises a chromosome (3,977,827 bp) and a megaplasmid (857,937 bp). This sequence information provides insight into the genome organization and biochemical pathways of OM-1. In addition, we identified lipid biosynthesis pathways in OM-1, paving the way to a better understanding of its biochemical characterization. Keywords: Nitratireductor, Biodiesel production, Short-chain fatty acid, Ester synthesis, Whole genome sequence