Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes

Abstract

Heterologous expression has been an important tool for structural and functionalcharacterization of proteins. The study of biophysical properties of ion channels,pumps and transporters has been possible thanks to their expression in Xenopuslaevisoocytes. Here we report the expression of two voltage gated channels, Kv1.1and Shaker, in X. laevisoocytes using a method for oocyte extraction, isolation, cul-ture, and microinjection adapted to the latitude and altitude conditions of Bogotá,Colombia.

Keywords

• Ovocito
• Xenopus Laevis
• Voltaje Clamp
• KV1.1
• Shaker
• Expresiónheteróloga