LzSCL9, a Novel GRAS Transcription Factor in Lanzhou Lily (<i>Lilium davidii</i> var. <i>unicolor</i>), Participates in Regulation of Trichokonins-Primed Heat Stress Tolerance
Xing Cao,
Liping Ding,
Jiahui Liang,
Yanrong Zhou,
Xiulan Chen,
Haiyan Li,
Tao Liu,
Wenxiu Yue,
Juanjuan Sui,
Liangbao Jiang,
Yulian Qian,
Dongdong Yang,
Bo Wang,
Hailing Zhang,
Ze Wu,
Xiaoyan Song
Affiliations
Xing Cao
College of Architecture, Yantai University, Yantai 264005, China
Liping Ding
Key Laboratory of Landscaping Agriculture, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Jiahui Liang
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Yanrong Zhou
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
Xiulan Chen
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
Haiyan Li
College of Architecture, Yantai University, Yantai 264005, China
Tao Liu
College of Architecture, Yantai University, Yantai 264005, China
Wenxiu Yue
College of Architecture, Yantai University, Yantai 264005, China
Juanjuan Sui
Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine, Biology and Food Engineering College, Fuyang Normal University, Fuyang 236037, China
Liangbao Jiang
College of Architecture, Yantai University, Yantai 264005, China
Yulian Qian
College of Architecture, Yantai University, Yantai 264005, China
Dongdong Yang
College of Life Science, Yantai University, Yantai 264005, China
Bo Wang
College of Life Science, Yantai University, Yantai 264005, China
Hailing Zhang
College of Life Science, Yantai University, Yantai 264005, China
Ze Wu
Key Laboratory of Landscaping Agriculture, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Xiaoyan Song
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
In our previous research, we found that trichokonins’ (TKs) employment improved the thermotolerance of the Lanzhou lily, a renowned edible crop species endemic to China that is relatively susceptible to high temperatures (HTs). Here, a novel Lanzhou lily GRAS gene, LzSCL9, was identified to respond to heat stress (HS) and HS+TKs treatment based on transcriptome and RT-qPCR analysis. TKs could improve the upregulation of LzSCL9 during long-term HS. The expression profile of LzSCL9 in response to HS with or without TKs treatment showed a significant positive correlation with LzHsfA2a-1, which was previously identified as a key regulator in TKs’ conferred resilience to HT. More importantly, overexpression of LzSCL9 in the lily enhanced its tolerance to HTs and silencing LzSCL9 in the lily reduced heat resistance. Taken together, this study identified the positive role of LzSCL9 in TK-induced thermotolerance, thereby preliminarily establishing a molecular mechanism on TKs regulating the thermostability of the Lanzhou lily and providing a new candidate regulator for plant heat-resistant breeding.