An Integrated Strategy for Nutraceuticals from <i>Haematoccus pluvialis</i>: From Cultivation to Extraction
Sanjeet Mehariya,
Neeta Sharma,
Angela Iovine,
Patrizia Casella,
Tiziana Marino,
Vincenzo Larocca,
Antonio Molino,
Dino Musmarra
Affiliations
Sanjeet Mehariya
Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa (CE), Italy
Neeta Sharma
ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Trisaia, SS Jonica 106, km 419 + 500, 75026 Rotondella (MT), Italy
Angela Iovine
Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa (CE), Italy
Patrizia Casella
ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Portici, P. Enrico Fermi, 1, 80055 Portici (NA), Italy
Tiziana Marino
Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa (CE), Italy
Vincenzo Larocca
ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Trisaia, SS Jonica 106, km 419 + 500, 75026 Rotondella (MT), Italy
Antonio Molino
ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Department of Sustainability-CR Portici, P. Enrico Fermi, 1, 80055 Portici (NA), Italy
Dino Musmarra
Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa (CE), Italy
The aim of this study was to develop an effective integrated cultivation system for Haematococcus pluvialis as a source of bioactive compounds such as astaxanthin, lutein, proteins, and fatty acids (FAs). The Chlorophyta H. pluvialis was cultivated in a vertical bubble column photobioreactor (VBC-PBR) under batch mode, allowing switching from green to red phase for astaxanthin induction. The combined effect of light intensity and nutrients on bioactive compound formation was investigated. Results showed that growth under lower nutrients availability and light intensity led to a higher concentration of biomass. Growth under high light intensity with an appropriate concentration of nitrate, sulfate, phosphate and magnesium led to ~85% and ~58% higher production of total carotenoids and fatty acids, respectively. Under high stress conditions, ~90% nitrate and phosphate consumption were observed.
