Análise conformacional por cálculos teóricos da distinctina, peptídeo antimicrobiano isolado de anuros da espécie Phyllomedusa distincta Conformational analysis by theoretical calculations of distinctin, an antimicrobial peptide isolated from Phyllomedusa distincta

Abstract

Various studies demonstrate that different frog species produce distinct classes of biologically active peptides. These peptides can act as alternative agents against pathogenic bacteria and fungi by membrane permeability. Although studies have recently demonstrated that this process is utterly related to the secondary structure adopted by the peptide (in this case, the a-helical structure) when in contact with the bacterial membrane, the detailed mechanism is still unknown. In this work we describe a conformational analysis of distinctin, a heterodimeric peptide isolated from the skin of Phyllomedusa distincta, an anuran found in the Brazilian Atlantic Forest. The study yielded a stable geometry with a high content of the a-helical structure both in chains 1 and 2 of distinctin, showing strong interaction between them.

Keywords

• Phyllomedusa distincta
• antimicrobial peptide
• conformational analysis