Erythroprotective Potential of Phycobiliproteins Extracted from <i>Porphyridium cruentum</i>
Rubria Lucía Peña-Medina,
Diana Fimbres-Olivarría,
Luis Fernando Enríquez-Ocaña,
Luis Rafael Martínez-Córdova,
Carmen Lizette Del-Toro-Sánchez,
José Antonio López-Elías,
Ricardo Iván González-Vega
Affiliations
Rubria Lucía Peña-Medina
Department of Scientific and Technological Research, University of Sonora, Blvd Luis Encinas y Reforma S/N, Col. Centro, Hermosillo 83000, Mexico
Diana Fimbres-Olivarría
Department of Scientific and Technological Research, University of Sonora, Blvd Luis Encinas y Reforma S/N, Col. Centro, Hermosillo 83000, Mexico
Luis Fernando Enríquez-Ocaña
Department of Scientific and Technological Research, University of Sonora, Blvd Luis Encinas y Reforma S/N, Col. Centro, Hermosillo 83000, Mexico
Luis Rafael Martínez-Córdova
Department of Scientific and Technological Research, University of Sonora, Blvd Luis Encinas y Reforma S/N, Col. Centro, Hermosillo 83000, Mexico
Carmen Lizette Del-Toro-Sánchez
Department of Research and Postgraduate in Food, University of Sonora, Blvd Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, Mexico
José Antonio López-Elías
Department of Scientific and Technological Research, University of Sonora, Blvd Luis Encinas y Reforma S/N, Col. Centro, Hermosillo 83000, Mexico
Ricardo Iván González-Vega
Department of Medical and Life Sciences, Cienega University Center (CUCIÉNEGA), University of Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Mexico
There are multiple associations between the different blood groups (ABO and RhD) and the incidence of oxidative stress-related diseases, such as certain carcinomas and COVID-19. Bioactive compounds represent an alternative to its prevention and treatment. Phycobiliproteins (PBP) are bioactive compounds present in the microalga Porphyridium cruentum and, despite its antioxidant activity, their inhibitory effect on hemolysis has not been reported. The aim of this work was to evaluate the erythroprotective potential of phycobiliproteins from P. cruentum in different blood groups. The microalga was cultured in F/2 medium under controlled laboratory conditions. Day 10 of culture was determined as the harvest point. The microalgal biomass was lyophilized and a methanolic (MetOH), Tris HCl (T-HCl), and a physiological solution (PS) ultrasound-assisted extraction were performed. Extract pigments were quantified by spectrophotometry. The antioxidant activity of the extracts was evaluated with the ABTS+•, DPPH•, and FRAP methods, finding that the main antioxidant mechanism on the aqueous extracts was HAT (hydrogen atom transfer), while for MetOH it was SET (single electron transfer). The results of the AAPH, hypotonicity, and heat-induced hemolysis revealed a probable relationship between the different antigens (ABO and RhD) with the antihemolytic effect, highlighting the importance of bio-directed drugs.
