Antimicrobial Activity of <i>Smilax china</i> L. Root Extracts against the Acne-Causing Bacterium, <i>Cutibacterium acnes,</i> and Its Active Compounds
Ji-Hae Joo,
Min-Hui Han,
Ja-I Kim,
Jong-Eun Kim,
Kyung-Hwan Jung,
Han Sun Oh,
Young Soo Chung,
Hyun Jin An,
Jae Duk Lee,
Gi-Seong Moon,
Hyang-Yeol Lee
Affiliations
Ji-Hae Joo
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Min-Hui Han
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Ja-I Kim
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Jong-Eun Kim
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Kyung-Hwan Jung
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Han Sun Oh
Beauty Cosmetic Co., Ltd., 274-14 Wonnamsandan-ro, Wonnam-myeon 32740, Eumseong-gun, Chungbuk, Republic of Korea
Young Soo Chung
Beauty Cosmetic Co., Ltd., 274-14 Wonnamsandan-ro, Wonnam-myeon 32740, Eumseong-gun, Chungbuk, Republic of Korea
Hyun Jin An
Yeomyung Biochem Co., Ltd., 7-4 Tabyeon 1-gil, Gangane-myeon, Heungdeok-gu, Cheongju-si 28171, Chungbuk, Republic of Korea
Jae Duk Lee
Yeomyung Biochem Co., Ltd., 7-4 Tabyeon 1-gil, Gangane-myeon, Heungdeok-gu, Cheongju-si 28171, Chungbuk, Republic of Korea
Gi-Seong Moon
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
Hyang-Yeol Lee
Division of Food Science and Biotechnology, Korea National University of Transportation, Chungju 27909, Chungbuk, Republic of Korea
The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. extracts for their potential use as active ingredients in cosmetics for the treatment of human skin acne. The high-performance liquid chromatography (HPLC) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC/QTOF/MS) methods were used to obtain the profile of secondary metabolites from the ethyl acetate-soluble fraction of the crude extract. Agar diffusion and resazurin-based broth microdilution assays were used to evaluate antimicrobial activity and minimum inhibitory concentrations (MIC), respectively. Among the 24 metabolites, quercetin, resveratrol, and oxyresveratrol were the most potent compounds against Cutibacterium acnes. Minimum inhibitory concentrations of quercetin, resveratrol, and oxyresveratrol were 31.25, 125, and 250 μg/mL, respectively.
