Department of Pharmaceutical Sciences, University of California, Irvine, United States; Department of Chemistry, University of California, Irvine, United States
Department of Pharmaceutical Sciences, University of California, Irvine, United States; Department of Organic Chemistry, Charles University, Prague, Czech Republic
Department of Pharmaceutical Sciences, University of California, Irvine, United States; Department of Molecular Biology and Biochemistry, University of California, Irvine, United States; Department of Chemistry, University of California, Irvine, United States
Optogenetic tools have revolutionized the study of receptor-mediated processes, but such tools are lacking for RNA-controlled systems. In particular, light-activated regulatory RNAs are needed for spatiotemporal control of gene expression. To fill this gap, we used in vitro selection to isolate a novel riboswitch that selectively binds the trans isoform of a stiff-stilbene (amino-tSS)–a rapidly and reversibly photoisomerizing small molecule. Structural probing revealed that the RNA binds amino-tSS about 100-times stronger than the cis photoisoform (amino-cSS). In vitro and in vivo functional analysis showed that the riboswitch, termed Werewolf-1 (Were-1), inhibits translation of a downstream open reading frame when bound to amino-tSS. Photoisomerization of the ligand with a sub-millisecond pulse of light induced the protein expression. In contrast, amino-cSS supported protein expression, which was inhibited upon photoisomerization to amino-tSS. Reversible photoregulation of gene expression using a genetically encoded RNA will likely facilitate high-resolution spatiotemporal analysis of complex RNA processes.
