Efficient and Stable Magnetic Chitosan-Lipase B from <i>Candida Antarctica</i> Bioconjugates in the Enzymatic Kinetic Resolution of Racemic Heteroarylethanols
Cristina Georgiana Spelmezan,
László Csaba Bencze,
Gabriel Katona,
Florin Dan Irimie,
Csaba Paizs,
Monica Ioana Toșa
Affiliations
Cristina Georgiana Spelmezan
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
László Csaba Bencze
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
Gabriel Katona
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
Florin Dan Irimie
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
Csaba Paizs
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
Monica Ioana Toșa
Biocatalysis and Biotransformation Research Center, Babeș-Bolyai University, Arany János 11, Cluj-Napoca 400028, Romania
Lipase B from Candida antarctica immobilized by covalent binding on sebacoyl-activated chitosan-coated magnetic nanoparticles proved to be an efficient biocatalyst (49.2−50% conversion in 3−16 h and >96% enantiomeric excess) for the enzymatic kinetic resolution of some racemic heteroarylethanols through transesterification with vinyl acetate. Under optimal conditions (vinyl acetate, n-hexane, 45 °C), the biocatalyst remains active after 10 cycles.
