Identification of genotype Trichinella spiralis using polymerase chain reaction

Abstract

Methods for interspecific and intraspecific differentiation of helminthes, iden-tification of genotype Trichinella spiralis using polymerase chain reaction are de-veloped. These methods detect DNA loci specific for genome T. spiralis and ena-bles to determine connection between natural and synanthropic trichinellosis in different biocenosis. Methods are based on interaction of synthesized oligonucleo-tide primers with DNA loci typical for this parasite genotype. The amplified frag-ment length is increasing. Electrophoresis of amplification products enables to vis-ualize the DNA fragments and find their molecular mass. The list of equipment and reagents is presented, the process of preparation of clinical trial materials, release of genomic DNA, preparation of reaction mixture for polymerase chain reaction, amplification and electrophoresis modes are described

Keywords

• trichinella spiralis
• method of polymerase chain reaction
• differ-entiation
• dna