A Vaccine with Multiple Receptor-Binding Domain Subunit Mutations Induces Broad-Spectrum Immune Response against SARS-CoV-2 Variants of Concern

Xu-Chen Hou; Hui-Fang Xu; Yang Liu; Peng Sun; Lin-Wei Ding; Jun-Jie Yue; Tian-Tian Wang; Xin Gong; Jun Wu; Bo Liu

doi:10.3390/vaccines10101653

Vaccines (Oct 2022)

A Vaccine with Multiple Receptor-Binding Domain Subunit Mutations Induces Broad-Spectrum Immune Response against SARS-CoV-2 Variants of Concern

Xu-Chen Hou,
Hui-Fang Xu,
Yang Liu,
Peng Sun,
Lin-Wei Ding,
Jun-Jie Yue,
Tian-Tian Wang,
Xin Gong,
Jun Wu,
Bo Liu

Affiliations

Xu-Chen Hou: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Hui-Fang Xu: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Yang Liu: School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China
Peng Sun: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Lin-Wei Ding: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Jun-Jie Yue: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Tian-Tian Wang: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Xin Gong: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Jun Wu: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Bo Liu: Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China

DOI: https://doi.org/10.3390/vaccines10101653
Journal volume & issue: Vol. 10, no. 10
p. 1653

Abstract

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With the emergence of more variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the immune evasion of these variants from existing vaccines, the development of broad-spectrum vaccines is urgently needed. In this study, we designed a novel SARS-CoV-2 receptor-binding domain (RBD) subunit (RBD5m) by integrating five important mutations from SARS-CoV-2 variants of concern (VOCs). The neutralization activities of antibodies induced by the RBD5m candidate vaccine are more balanced and effective for neutralizing different SARS-CoV-2 VOCs in comparison with those induced by the SARS-CoV-2 prototype strain RBD. Our results suggest that the RBD5m vaccine is a good broad-spectrum vaccine candidate able to prevent disease from several different SARS-CoV-2 VOCs.

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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