Inflammatory markers in Huntington's disease plasma—A robust nanoLC–MRM-MS assay development

Abstract

The development of an MRM assay for the measurements of six inflammatory markers is presented. We report a robust and sensitive quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing. The assay has a dynamic range with 4 orders of magnitude and the LOQs are in the attomolar range. We used plasma from Huntington's disease gene carriers and healthy controls to compare our MRM method with antibody based methods. Importantly, we found a good agreement between assays for the measurement of C-reactive protein, in contrast to complement component 3 and complement factor H.

Keywords

• Multiple reaction monitoring
• Complement components
• C-reactive protein
• Huntington's disease