JIIS: Jurnal Ilmiah Ibnu Sina (Mar 2018)
SCREENING FITOKIMIA, AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA PADA BUAH JERUK LEMON(Citrus limon) DAN JERUK NIPIS (Citrus aurantiifolia)
Abstract
The desire to live healthy by eating natural foods and drinks into the lifestyle of the community. Orange becomes one of the fruits that become functional food to maintain and maintain health. The purpose of this research is to know the difference of antioxidant and antimicrobial activity on Citrus limon and Citrus aurantiifolia. The research method is laboratory experimental research with descriptive analysis. This research was conducted in February-April 2017 at the Laboratory of Plant Biological Microbiology and Plant Chemistry Department of Biology State University of Malang. Phytochemical screening by color reaction method, total phenol with Folin Ciocalteu method, antioxidant activity with DPPH method and antimicrobial activity with disc method. Screening results show the presence of saponins and alkaloids but there are no flavonoids, terpenoids and tannins. Total phenol test showed total phenol content in Lemon (Citrus limon) of 110,25 mg GAE / 100ml while in Lime (Citrus aurantiifolia) 116,5 mg GAE / 100ml. The antioxidant activity of Lemon Citrus (Citrus limon) 49.593 g / ml and Lime (Citrus aurantiifolia) 49.589g / ml. Antimicrobial activity test obtained the highest zone of resistance at 100% concentration of each citrus fruit. The conclusion of this study is that there is a difference of antioxidant and antimicrobial activity in both oranges, where the lemon fruits (C.limon) antioxidant activity is higher than and Lime (C. aurantiifolia), while the antimicrobial activity of lemon (C. aurantiifolia) is higher Rather than lemon (C.limon).
