Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9

Mathilde Mathieu; Nathalie Névo; Mabel Jouve; José Ignacio Valenzuela; Mathieu Maurin; Frederik J. Verweij; Roberta Palmulli; Danielle Lankar; Florent Dingli; Damarys Loew; Eric Rubinstein; Gaëlle Boncompain; Franck Perez; Clotilde Théry

doi:10.1038/s41467-021-24384-2

Nature Communications (Jul 2021)

Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9

Mathilde Mathieu,
Nathalie Névo,
Mabel Jouve,
José Ignacio Valenzuela,
Mathieu Maurin,
Frederik J. Verweij,
Roberta Palmulli,
Danielle Lankar,
Florent Dingli,
Damarys Loew,
Eric Rubinstein,
Gaëlle Boncompain,
Franck Perez,
Clotilde Théry

Affiliations

Mathilde Mathieu: INSERM U932, Institut Curie Centre de Recherche, PSL Research University
Nathalie Névo: INSERM U932, Institut Curie Centre de Recherche, PSL Research University
Mabel Jouve: CNRS UMR3215, Institut Curie, PSL Research University
José Ignacio Valenzuela: CNRS UMR144, Institut Curie, PSL Research University
Mathieu Maurin: INSERM U932, Institut Curie Centre de Recherche, PSL Research University
Frederik J. Verweij: Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266
Roberta Palmulli: Université de Paris
Danielle Lankar: INSERM U932, Institut Curie Centre de Recherche, PSL Research University
Florent Dingli: Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique
Damarys Loew: Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique
Eric Rubinstein: Sorbonne Université, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, CIMI-Paris
Gaëlle Boncompain: CNRS UMR144, Institut Curie, PSL Research University
Franck Perez: CNRS UMR144, Institut Curie, PSL Research University
Clotilde Théry: INSERM U932, Institut Curie Centre de Recherche, PSL Research University

DOI: https://doi.org/10.1038/s41467-021-24384-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 18

Abstract

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Extracellular vesicles (EVs) play a role in intercellular communication, however the precise biogenesis of different populations of EVs are not clear. Here, the authors follow the intracellular trafficking of two proteins before their secretion in EVs and report the biogenesis and protein markers of EV subtypes: ectosomes budding from the plasma membrane as well as exosomes from late endosomes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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