Ukrainian Journal of Forest and Wood Science (Nov 2021)
Effect of rutin-ammonium complex on the physiological state of Scots pine seedlings
Abstract
In the plant organism, phenolic compounds have a non-specific effect on the processes of morphogenesis and perform a wide range of regulatory and protective functions. Of particular interest are the processes associated with the complex formation of flavonoids as a result of their interaction with ammonium forms of nitrogen. Polar compounds that are formed in tissues by chemical transformation are quite mobile in soil solutions and exhibit high biological activity. The properties of phenol-ammonium complexes are of considerable interest in terms of morphogenesis, physiology of resistance, and in the system of interaction of plants with soil microorganisms. This is also extremely important in the production of high-quality planting material that is resistant to unfavourable factors. The effect of the phenol-ammonium complex was studied on seeds and seedlings of Scots pine. Quantitative indicators of germination energy and similarity were determined by the seed germination method. Biochemical profiling of seedling tissue extracts was performed by high-performance thin-layer chromatography. It has been experimentally confirmed that rutin (quercetin3-o-rutinoside) after interaction with a 10% aqueous solution of ammonia forms a complex of substances, among which polar products were detected by chromatography, which potentially affect growth regulation. At a total concentration of 15 mg/l, these substances significantly increased germination energy and germinating ability. In pine seedlings, they stimulated the growth of roots and shoots. The effect of the complex of organic compounds on seedlings depended on the concentration, duration of seed treatment, and had a prolonged effect. The resulting phenol-ammonium complex at a concentration of 10-15 mg/l contributed to an increase in the amount of chlorophylls and carotenoids in the tissues of seedlings, and at 20-40 mg/l it increased the content of phenolic synthesis products
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