Association Between Serum Thyroid-Stimulating Hormone Levels and Salivary Microbiome Shifts

Ting Dong; Ting Dong; Ting Dong; Ting Dong; Fen Zhao; Fen Zhao; Fen Zhao; Fen Zhao; Keyong Yuan; Keyong Yuan; Keyong Yuan; Keyong Yuan; Xiaohan Zhu; Xiaohan Zhu; Xiaohan Zhu; Xiaohan Zhu; Ningjian Wang; Fangzhen Xia; Yingli Lu; Zhengwei Huang; Zhengwei Huang; Zhengwei Huang; Zhengwei Huang

doi:10.3389/fcimb.2021.603291

Frontiers in Cellular and Infection Microbiology (Feb 2021)

Association Between Serum Thyroid-Stimulating Hormone Levels and Salivary Microbiome Shifts

Ting Dong,
Ting Dong,
Ting Dong,
Ting Dong,
Fen Zhao,
Fen Zhao,
Fen Zhao,
Fen Zhao,
Keyong Yuan,
Keyong Yuan,
Keyong Yuan,
Keyong Yuan,
Xiaohan Zhu,
Xiaohan Zhu,
Xiaohan Zhu,
Xiaohan Zhu,
Ningjian Wang,
Fangzhen Xia,
Yingli Lu,
Zhengwei Huang,
Zhengwei Huang,
Zhengwei Huang,
Zhengwei Huang

Affiliations

Ting Dong: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Ting Dong: National Clinical Research Center for Oral Diseases, Shanghai, China
Ting Dong: Shanghai Key Laboratory of Stomatology, Shanghai, China
Ting Dong: Shanghai Research Institute of Stomatology, Shanghai, China
Fen Zhao: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Fen Zhao: National Clinical Research Center for Oral Diseases, Shanghai, China
Fen Zhao: Shanghai Key Laboratory of Stomatology, Shanghai, China
Fen Zhao: Shanghai Research Institute of Stomatology, Shanghai, China
Keyong Yuan: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Keyong Yuan: National Clinical Research Center for Oral Diseases, Shanghai, China
Keyong Yuan: Shanghai Key Laboratory of Stomatology, Shanghai, China
Keyong Yuan: Shanghai Research Institute of Stomatology, Shanghai, China
Xiaohan Zhu: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Xiaohan Zhu: National Clinical Research Center for Oral Diseases, Shanghai, China
Xiaohan Zhu: Shanghai Key Laboratory of Stomatology, Shanghai, China
Xiaohan Zhu: Shanghai Research Institute of Stomatology, Shanghai, China
Ningjian Wang: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Fangzhen Xia: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Yingli Lu: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Zhengwei Huang: Department of Endodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Zhengwei Huang: National Clinical Research Center for Oral Diseases, Shanghai, China
Zhengwei Huang: Shanghai Key Laboratory of Stomatology, Shanghai, China
Zhengwei Huang: Shanghai Research Institute of Stomatology, Shanghai, China

DOI: https://doi.org/10.3389/fcimb.2021.603291
Journal volume & issue: Vol. 11

Abstract

Read online

High serum thyroid-stimulating hormone (TSH) levels are linked to many metabolic disorders, but the effects of TSH levels on the oral microbiota are still largely unknown. This study aimed to explore the association between the salivary microbiome in adults and serum TSH levels. Saliva and fasting blood samples were obtained from a health census conducted in Southeast China. All participants were divided according to serum TSH levels. The microbial genetic profiles and changes were acquired by 16S rDNA sequencing and bioinformatics analysis. Relevant anthropometric and biochemical measurements such as insulin resistance, blood lipids, and body composition were evaluated with laboratory tests and physical examinations. The salivary microbiome in individuals with higher TSH level showed significantly higher taxa diversity. Principal coordinates analysis and partial least squares discriminant analysis showed distinct clustering in the Abnormal and Normal Groups (Adonis, P=0.0320). Granulicatella was identified as a discriminative genus for comparison of the two groups. Fasting serum insulin, Homeostatic Model Assessment for Insulin Resistance, and hemoglobin A1 were elevated in the Abnormal Group (P<0.05), showing the presence of insulin resistance in individuals with abnormal higher serum TSH levels. Distance-based redundancy analysis revealed the association of this distinctive difference with salivary microbiome. In conclusion, shifts in microbial profile were observed in the saliva of individuals with different serum TSH levels, and insulin resistance may play an important role in the biochemical and microbial alteration.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

About the journal

Abstract

Keywords