Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks
Valesca Weingartner Montibeller,
Luciana Porto de Souza Vandenberghe,
Antonella Amore,
Carlos Ricardo Soccol,
Leila Birolo,
Roberto Vinciguerra,
Denise Naomi Xavier Salmon,
Michele Rigon Spier,
Vincenza Faraco
Affiliations
Valesca Weingartner Montibeller
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; Brazil
Luciana Porto de Souza Vandenberghe
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; Brazil
Antonella Amore
Department of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; Italy
Carlos Ricardo Soccol
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; Brazil
Leila Birolo
Department of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; Italy
Roberto Vinciguerra
Department of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; Italy
Denise Naomi Xavier Salmon
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; Brazil
Michele Rigon Spier
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, Zip Code 81531-990 Curitiba, Brazil; Brazil
Vincenza Faraco
Department of Chemical Sciences, University of Naples “Federico II,” Complesso Universitario Monte S. Angelo, via Cintia, 4 80126 Naples, Italy; Italy
This manuscript describes the analysis of xylanase production by Aspergillus niger NRRL 328 in solid state fermentation (SSF) of soybean husks. A maximum value of extracellular xylanase activity of approximately 950 U g-1 was achieved after 96 h. Proteomic analyses performed on the enzymatic mixture responsible for the maximum value of xylanase activity in SSF revealed the presence of two xylanases. This xylanolytic mixture was partially purified and characterized. It followed Michaelis-Menten kinetics towards xylan, with a KM of 7.92 ±0.97 mg xylan/mL and a Vmax of 262.2 ± 27.8 g L-1 s-1. The optimum pH for the enzyme is 5.3, and the optimal temperature is 50 °C. The enzyme retains 100% of its activity at 40 °C for at least 1 month. It shows very high stability in a broad pH range, with a half-life of 40 days at pH 5.3, pH 6.0, pH 6.5, pH 7.0, and pH 8.0.
