Analysis of ovine transferrin polymorphisms and their relationship with blood metabolite variation in Makoei sheep breed

Abstract

In order to determine polymorphism of ovine transferrin and its association with blood metabolite in Makoei sheep, blood samples were collected from 576 male and female lambs using two types of Venoject (with or without EDTA). Plasma and serum were then separated and kept in -20₀C. Transferrin polymorphisms were determined by vertical electrophoresis of polyacrylamid gels. Results indicated a total of 24 genotypes involving the 10 alleles C, B, D, A, E, G, L, K, M and O, ordered on the basis of their frequencies. Among these the C allele was the most frequent (0.29) and the M allele was the rarest (0.004). Association of the transferrin polymorphisms with triglyceride and total protein blood was very significant (P<0.01). So that the AA genotype the most amount cholesterol and total protein blood and AQ genotype has the lowest amount cholesterol and total protein blood. But, there was no significant association between genotypes of various transferrin proteins with cholesterol and glucose blood. Further investigation should be on the molecular level of transferrin gene, using treatment samples.

Keywords

• makoei sheep
• polymorphism
• transferrin
• blood metabolites