Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells
Shin La Shu,
Lakshmi Bhargavi Paruchuru,
Neil Quanwei Tay,
Yen Leong Chua,
Adeline Shen Yun Foo,
Chris Maolin Yang,
Ka Hang Liong,
Esther Geok Liang Koh,
Angeline Lee,
Hovav Nechushtan,
Ehud Razin,
David Michael Kemeny
Affiliations
Shin La Shu
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Lakshmi Bhargavi Paruchuru
Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University of Jerusalem, Jerusalem 91120, Israel
Neil Quanwei Tay
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Yen Leong Chua
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Ka Hang Liong
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Esther Geok Liang Koh
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Angeline Lee
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore
Hovav Nechushtan
Sharett Institute of Oncology, Hadassah Hebrew University Medical Center, Hebrew University of Jerusalem, POB 12000 Jerusalem, Israel; Corresponding author
Ehud Razin
NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore; Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University of Jerusalem, Jerusalem 91120, Israel; Corresponding author
David Michael Kemeny
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore; Corresponding author
Summary: The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (≈30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs. : Molecular Mechanism of Behavior; Immunology; Immune Response; Model Organism Subject Areas: Molecular Mechanism of Behavior, Immunology, Immune Response, Model Organism
